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| Two-dimensional resolution of the mitochondrial proteome from Solanum tuberosum (potato) by Blue-native / Tricine SDS polyacrylamide gel electrophoresis (Jänsch et al. 1996, Plant J. 9, 357-368). Arrows indicate identified proteins (a list of identified proteins is given below). Identities of the separated protein complexes (from left to right): NADH dehydrogenase (~ 1000 kDa, >30 subunits), HSP60 complex (~ 750 kDa, 14mer of the HSP60 protein), F0F1 ATP synthase complex (~ 580 kDa, 13 subunits), cytochrome c reductase (~ 480 kDa, 10 subunits), F1 ATP synthase (~ 350 kDa, 6 subunits), cytochrome c oxidase (~ 230 kDa, 10 subunits).
All 10 subunits of cytochrome c reductase from potato were identified (for review see Braun and Schmitz 1995, J. Bioenerg. Biomembr. 27, 423-436).
See also: Mitochondrial protein complexes of Arabidopsis |
List of identified proteins
(last update: Nov. 2002)
Proteins were identified by Edman Degradation (ED) or Immunostaining (IgG). Sequences determined are N-terminal sequences.
Protein No | Identity | Accession | Identification | Sequences determined | 1 | Complex I, 18 kDa subunit | P80268 | ED | ASEALVEIKPGEIGM... | 2 | ATP synthase (F0-part), 27 kDa subunit | P80496 | ED | AKEAAAPTTLKGDQVL... | 3 | ATP synthase (F0-part), ATP9 subunit | 1915346B | ED | MLEGA KLMGAGA ... | 4 | ATP synthase (F0-part), 6 kDa subunit | P80497 | ED | MRQFDPWPVFFRREW... | 5 | Complex III, FeS subunit | P37841 | ED, IgG | SNSVSPAHQLGLVSD... | 6 | ATP synthase (F1-part), delta' subunit | P80504 | ED | ATDLQAGYVADSNFT... | 7 | ATP synthase (F1-part), ATPase inhib. subunit | 2113194A | ED | XDSKGRIFSEEERAK... | 9 | Complex IV, Cox II subunit | P80498 | ED | DAAEPWQLGFQDAAT... | 10 | Complex IV, subunit Vb | P80499 | ED | TVSENVVKKVEDVMP... | 11 | Complex IV, subunit VIII (Vb) | P80500 | ED | AGXXXAHXXYKGPSV... | 12 | Porin (POM34) | P42055 | ED, IgG | GKGPGLYTEIGKKAR... | 13 | HSP60 | P80502 | ED | AAKDIKFGVEARGLM... | 14 | formate dehydrogenase | CAA79702 | ED | LQASPGPKKIVGVFY... | 16 | glycine decarboxylase, L-subunit | P80503 | ED | ASGSDENDVVVIGGG... | 20 | Complex III, beta-MPP subunit | A48529
X80235 | ED, IgG
ED, IgG | STSITNPSNSSSLPS...
SASAAVAATSSSTPA... | 21 | Complex III, alpha-MPP subunit | P29677 | ED, IgG | SSSAAVATXPSGGL... | 22 | Complex III, cytochrome b | P29757 | ED, IgG | TIRNQRLSLLKQPIS... | 23 | Complex III, cytochrome c1 | P29610 | ED, IgG | DEAEHGLEXPSYPXP... | 24 | Complex III, 14 kDa subunit | P48502 | IgG | blocked | 25 | Complex III, Hinge subunit | P48504 | ED, IgG | SDEEVVDPKATLEVS... | 26 | Complex III, 8.2 kDa subunit | P46269 | ED | GKQPVKLKAVVYAIS... | 27 | Complex III, 8.0 kDa subunit | P46270 | ED | MESAARRSGGGVLEG... | 28 | Complex III, 6.7 kDa subunit | P48505 | ED | TSPAAAGNGLFKFLR... | 29 | NAD-dep. malate dehydrogenase | Q8L5A6 | ED | XXXSAPER KVAVLGAAG... | 30 | Porin (POM34) | P42055 | ED | GKGPGLYTEIGKKAR... | 31 | 12 kDa subunit, complex I | (At1g67350) | ED | GFIMEFAENLIRRLME... | 32 | 8 kDa subunit, complex I | (At2g31490) | ED | GGGMEYNKNKXIEE... |
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